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OfflineSmokeSomeHash
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what to do with free mycellium
    #692853 - 11/09/13 08:05 PM (10 years, 4 months ago)

Got a lil filter bag with a small brick of myc and a couple shrooms growing today.

Wonderin if i should put it in a terrarium or pick everything and spawn to more?

Been so long since i grew shrooms.

Maybe make a spore print or lc?


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InvisibleDeadkndys420
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Re: what to do with free mycellium [Re: SmokeSomeHash] * 2
    #692854 - 11/09/13 08:08 PM (10 years, 4 months ago)

Agar and isolate.

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OfflineSmokeSomeHash
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Re: what to do with free mycellium [Re: Deadkndys420]
    #692859 - 11/09/13 08:22 PM (10 years, 4 months ago)

Ive never done agar.

Ive heard its best. Do the petri dish and gtg transfers in like quart jars if i remember right?

I dont have a hood though. Just a cheap lil glovebox i made.


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InvisibleDeadkndys420
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Re: what to do with free mycellium [Re: SmokeSomeHash]
    #692862 - 11/09/13 08:47 PM (10 years, 4 months ago)

I have heard people using glove boxes (built right) without any problems.

There are many ways to colonize agar.A drop from a spore syringe or LC,a single colonized piece of grain,wedge of my mycelium from another petri dish, etc.

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OfflineSmokeSomeHash
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Re: what to do with free mycellium [Re: Deadkndys420]
    #692910 - 11/10/13 07:14 AM (10 years, 4 months ago)

My concern is i have no agar and im pressed for time.

Ill prolly pick the first flush today.

Ive made lcs before. Maybe i take a biopsy of a shroom i pick and clone it to an lc then use that to start agar?

Im just gonna need to order stuff...sigh

Just tryin to work with what ive got for now...

I have a lot of coir.


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OfflineNameInUse
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Re: what to do with free mycellium [Re: SmokeSomeHash]
    #692911 - 11/10/13 07:24 AM (10 years, 4 months ago)

Agar is the shit. You could simply save a shroom and reanimate that tissue later.
I started off this way using a simple SAB and Lysol. Then, once you isolate myc, transfer to WBS, and spawn with coco.


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But then an even greater force emerged: The un! And the un un-nazi'd the world! Forever!

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Re: what to do with free mycellium [Re: NameInUse]
    #692913 - 11/10/13 07:48 AM (10 years, 4 months ago)

i used to do a lot of agar work in a tiny closet in and old house i lived in. tiny closet! id go in there and spray a shitload of lysol and close the door. come back half an hour later and get to work, and do everything extremely fast. i rarely ever got a contamination.

if i were you OP, i would fruit out what you have since its in the fruiting stage, have your agar ready to use, and find a few nice mushrooms to clone. tissue transfers to the agar, grow it out, isolate if you want, transfer to grain, grow it out, then G2G to jars/bags.

good times. i miss growing mushrooms.

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OfflineSham87
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Re: what to do with free mycellium [Re: CrushNazT]
    #693002 - 11/10/13 05:33 PM (10 years, 4 months ago)

Anyone have a spare spore print?


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...once in a while you get shown the light in the strangest of places if you look at it right...


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OfflineSmokeSomeHash
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Re: what to do with free mycellium [Re: CrushNazT]
    #693007 - 11/10/13 05:47 PM (10 years, 4 months ago)

Quote:

CrushNazT said:
if i were you OP, i would fruit out what you have since its in the fruiting stage, have your agar ready to use, and find a few nice mushrooms to clone. tissue transfers to the agar, grow it out, isolate if you want, transfer to grain, grow it out, then G2G




Thats pretty much what i want to do but i need to order agar and dont know how long itll take.
Do tissue samples have to be off a fresh picked shroom?

Worst case scenario ill just spore print.


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OfflineNameInUse
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Re: what to do with free mycellium [Re: SmokeSomeHash]
    #693011 - 11/10/13 06:26 PM (10 years, 4 months ago)

No, you can reanimate dry tissue. The inside of the stipe is what I prefer.


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But then an even greater force emerged: The un! And the un un-nazi'd the world! Forever!

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InvisibleCrushNazT
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Re: what to do with free mycellium [Re: Sham87] * 1
    #693065 - 11/10/13 10:48 PM (10 years, 4 months ago)

Quote:

Sham87 said:
Anyone have a spare spore print?




yea. i've got a few i could send you. pm me if you want

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