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 SmokeSomeHash
Littlest pee pee on da block
Registered: 02/26/13
Posts: 1,555
Loc: murica 
Last seen: 10 years, 7 months
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 what to do with free mycellium 
#692853 - 11/09/13 08:05 PM (12 years, 10 months ago) |
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Got a lil filter bag with a small brick of myc and a couple shrooms growing today.
Wonderin if i should put it in a terrarium or pick everything and spawn to more?
Been so long since i grew shrooms.
Maybe make a spore print or lc?
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 Deadkndys420
Registered: 08/28/12
Posts: 8,720
Loc: █████
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Re: what to do with free mycellium [Re: SmokeSomeHash] 2
#692854 - 11/09/13 08:08 PM (12 years, 10 months ago) |
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Agar and isolate.
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SmokeSomeHash
Littlest pee pee on da block
Registered: 02/26/13
Posts: 1,555
Loc: murica
Last seen: 10 years, 7 months
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Re: what to do with free mycellium [Re: Deadkndys420]
#692859 - 11/09/13 08:22 PM (12 years, 10 months ago) |
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Ive never done agar.
Ive heard its best. Do the petri dish and gtg transfers in like quart jars if i remember right?
I dont have a hood though. Just a cheap lil glovebox i made.
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Deadkndys420
Registered: 08/28/12
Posts: 8,720
Loc: █████
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Re: what to do with free mycellium [Re: SmokeSomeHash]
#692862 - 11/09/13 08:47 PM (12 years, 10 months ago) |
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I have heard people using glove boxes (built right) without any problems.
There are many ways to colonize agar.A drop from a spore syringe or LC,a single colonized piece of grain,wedge of my mycelium from another petri dish, etc.
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SmokeSomeHash
Littlest pee pee on da block
Registered: 02/26/13
Posts: 1,555
Loc: murica
Last seen: 10 years, 7 months
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Re: what to do with free mycellium [Re: Deadkndys420]
#692910 - 11/10/13 07:14 AM (12 years, 10 months ago) |
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My concern is i have no agar and im pressed for time.
Ill prolly pick the first flush today.
Ive made lcs before. Maybe i take a biopsy of a shroom i pick and clone it to an lc then use that to start agar?
Im just gonna need to order stuff...sigh
Just tryin to work with what ive got for now...
I have a lot of coir.
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NameInUse
Strange
Registered: 10/30/12
Posts: 355
Last seen: 10 years, 10 months
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Re: what to do with free mycellium [Re: SmokeSomeHash]
#692911 - 11/10/13 07:24 AM (12 years, 10 months ago) |
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Agar is the shit. You could simply save a shroom and reanimate that tissue later.
I started off this way using a simple SAB and Lysol. Then, once you isolate myc, transfer to WBS, and spawn with coco.
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But then an even greater force emerged: The un! And the un un-nazi'd the world! Forever!
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CrushNazT
Registered: 07/23/13
Posts: 927
Loc: Colorado
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Re: what to do with free mycellium [Re: NameInUse]
#692913 - 11/10/13 07:48 AM (12 years, 10 months ago) |
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i used to do a lot of agar work in a tiny closet in and old house i lived in. tiny closet! id go in there and spray a shitload of lysol and close the door. come back half an hour later and get to work, and do everything extremely fast. i rarely ever got a contamination.
if i were you OP, i would fruit out what you have since its in the fruiting stage, have your agar ready to use, and find a few nice mushrooms to clone. tissue transfers to the agar, grow it out, isolate if you want, transfer to grain, grow it out, then G2G to jars/bags.
good times. i miss growing mushrooms.
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Sham87
マリオ
Registered: 03/20/12
Posts: 7,626
Last seen: 4 months, 16 days
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Re: what to do with free mycellium [Re: CrushNazT]
#693002 - 11/10/13 05:33 PM (12 years, 10 months ago) |
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Anyone have a spare spore print?
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...once in a while you get shown the light in the strangest of places if you look at it right...
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SmokeSomeHash
Littlest pee pee on da block
Registered: 02/26/13
Posts: 1,555
Loc: murica
Last seen: 10 years, 7 months
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Re: what to do with free mycellium [Re: CrushNazT]
#693007 - 11/10/13 05:47 PM (12 years, 10 months ago) |
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CrushNazT said:
if i were you OP, i would fruit out what you have since its in the fruiting stage, have your agar ready to use, and find a few nice mushrooms to clone. tissue transfers to the agar, grow it out, isolate if you want, transfer to grain, grow it out, then G2G
Thats pretty much what i want to do but i need to order agar and dont know how long itll take.
Do tissue samples have to be off a fresh picked shroom?
Worst case scenario ill just spore print.
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NameInUse
Strange
Registered: 10/30/12
Posts: 355
Last seen: 10 years, 10 months
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Re: what to do with free mycellium [Re: SmokeSomeHash]
#693011 - 11/10/13 06:26 PM (12 years, 10 months ago) |
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No, you can reanimate dry tissue. The inside of the stipe is what I prefer.
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But then an even greater force emerged: The un! And the un un-nazi'd the world! Forever!
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CrushNazT
Registered: 07/23/13
Posts: 927
Loc: Colorado
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Re: what to do with free mycellium [Re: Sham87] 1
#693065 - 11/10/13 10:48 PM (12 years, 10 months ago) |
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Sham87 said:
Anyone have a spare spore print?
yea. i've got a few i could send you. pm me if you want
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