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 jmello
Stranger
Registered: 01/20/10
Posts: 8
Last seen: 5 years, 2 months
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 I'm curious but lost 
#538954 - 03/22/11 02:26 PM (13 years, 1 month ago) |
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Can anyone point me to a good link regarding clone propagation on agar? I can't find a detailed tek?? Thanks for any help 
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the man
Registered: 04/20/08
Posts: 825
Last seen: 11 years, 2 months
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 Re: I'm curious but lost [Re: jmello]
#538957 - 03/22/11 02:33 PM (13 years, 1 month ago) |
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are you talking about microprop? you will have better luck with traditional methods. agar although looks cool creates a whole host of problems for a plant clone.
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KaptKid
Spaced Pirate
 
Registered: 04/20/08
Posts: 5,615
Loc: Bright Side of the Sun
Last seen: 4 months, 23 days
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 Re: I'm curious but lost [Re: jmello]
#538960 - 03/22/11 02:39 PM (13 years, 1 month ago) |
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Someone tried this before but faded away But there is a thread on it.
Hopefuly Magash will jump in on this one.
--------------------
Child of the 60's, Tripping ever sence.
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jmello
Stranger
Registered: 01/20/10
Posts: 8
Last seen: 5 years, 2 months
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Re: I'm curious but lost [Re: KaptKid]
#539013 - 03/22/11 07:29 PM (13 years, 1 month ago) |
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Thanks for the replies guys....i mainly want to read up on it....i have a history of trying tough things
Also I can fit a microprop setup in a very small place...i like stealth 
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Shroomofdoom
Menace 2 Society
Registered: 10/28/10
Posts: 401
Loc: Dark side of the moon
Last seen: 12 years, 1 month
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Re: I'm curious but lost [Re: jmello]
#539015 - 03/22/11 07:36 PM (13 years, 1 month ago) |
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I know this is off topic, but are you from Michigan? I had a friend named Justin Mellow (which your name reminds me of) that was from Michigan so im just wondering if thats where you got your name.
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 maryanne3087
Stranger
Registered: 06/27/10
Posts: 1,111
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Re: I'm curious but lost [Re: jmello]
#539141 - 03/23/11 06:10 AM (13 years, 1 month ago) |
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I have this saved on my computer. It was posted at another forum.
INFLUENCE OF CULTIVAR, EXPLANT SOURCE AND PLANT GROWTH
REGULATOR ON CALLUS INDUCTION AND PLANT REGENERATION
OF CANNABIS SATIVA L.
MATERIALS AND METHODS
Seeds of five cultivars of Cannabis sativa L. were
obtained from the Institute of Natural Fibres (Poznan´ ,
Poland): Silesia, Fibrimon-24, Novosadska, Juso-15
and Fedrina-74. Seedlings measuring ~10 cm, grown
in a growth chamber at 22°C under a 12 h photoperiod
were sterilized in 5% calcium hypochlorite for 6, 8 and
15 min and thoroughly rinsed in sterile water. Explants
*e-mail: ajar@igr.poznan.pl
Abbreviations: 2,4-D – dichlorophenoxyacetic acid; KIN – 6-furfurylaminopurine;
NAA – 1-naphthaleneacetic acid; DICAMBA – 3,6-
dichloro-o-anisic acid.
PL ISSN 0001-5296 © Polish Academy of Sciences, Cracow 2005
of young leaves, petioles, internodes and axillary buds
were implanted in Petri dishes (90 × 15 mm) containing
MS basal medium (Murashige and Skoog, 1962) supplemented
with various concentrations of plant growth
substances: 2,4-D, DIC, KIN and NAA (Tab. 1). Growth
regulators were added after the media were autoclaved.
Cultures were kept in darkness at 24°C for 2–3
weeks. Callus was excised from the original explants,
cut into ~0.5 cm3 pieces and transferred every 3 weeks
on the same fresh medium and incubated in a growth
chamber at 22°C under a 16 h photoperiod (~2000 lx).
For root formation, regenerated plantlets ~2 cm high
were excised from callus and cultured on MS basal
medium supplemented with 1.0 mg l-1 IAA and 1.0 mg
l-1 NAA. Rooted plants were transferred to soil and
grown in a greenhouse.
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jmello
Stranger
Registered: 01/20/10
Posts: 8
Last seen: 5 years, 2 months
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Re: I'm curious but lost [Re: maryanne3087]
#539359 - 03/23/11 07:09 PM (13 years, 30 days ago) |
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Quote:
Shroomofdoom said:
I know this is off topic, but are you from Michigan? I had a friend named Justin Mellow (which your name reminds me of) that was from Michigan so im just wondering if thats where you got your name.
Sorry man not me  I was high when I came up with that name 
Quote:
maryanne3087 said:
I have this saved on my computer. It was posted at another forum.
INFLUENCE OF CULTIVAR, EXPLANT SOURCE AND PLANT GROWTH
REGULATOR ON CALLUS INDUCTION AND PLANT REGENERATION
OF CANNABIS SATIVA L.
MATERIALS AND METHODS
Seeds of five cultivars of Cannabis sativa L. were
obtained from the Institute of Natural Fibres (Poznan´ ,
Poland): Silesia, Fibrimon-24, Novosadska, Juso-15
and Fedrina-74. Seedlings measuring ~10 cm, grown
in a growth chamber at 22°C under a 12 h photoperiod
were sterilized in 5% calcium hypochlorite for 6, 8 and
15 min and thoroughly rinsed in sterile water. Explants
*e-mail: ajar@igr.poznan.pl
Abbreviations: 2,4-D – dichlorophenoxyacetic acid; KIN – 6-furfurylaminopurine;
NAA – 1-naphthaleneacetic acid; DICAMBA – 3,6-
dichloro-o-anisic acid.
PL ISSN 0001-5296 © Polish Academy of Sciences, Cracow 2005
of young leaves, petioles, internodes and axillary buds
were implanted in Petri dishes (90 × 15 mm) containing
MS basal medium (Murashige and Skoog, 1962) supplemented
with various concentrations of plant growth
substances: 2,4-D, DIC, KIN and NAA (Tab. 1). Growth
regulators were added after the media were autoclaved.
Cultures were kept in darkness at 24°C for 2–3
weeks. Callus was excised from the original explants,
cut into ~0.5 cm3 pieces and transferred every 3 weeks
on the same fresh medium and incubated in a growth
chamber at 22°C under a 16 h photoperiod (~2000 lx).
For root formation, regenerated plantlets ~2 cm high
were excised from callus and cultured on MS basal
medium supplemented with 1.0 mg l-1 IAA and 1.0 mg
l-1 NAA. Rooted plants were transferred to soil and
grown in a greenhouse.
That actually helps a lot maryanne thank you I couldn't even really find out what micropropagation involved
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