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Offlinejmello
Stranger

Registered: 01/21/10
Posts: 8
Last seen: 1 year, 4 months
I'm curious but lost
    #538954 - 03/22/11 04:26 PM (5 years, 8 months ago)

Can anyone point me to a good link regarding  clone propagation on agar? I can't find a detailed tek?? Thanks for any help :smile:


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Offlinethe man

Registered: 04/20/08
Posts: 825
Last seen: 3 years, 10 months
Re: I'm curious but lost [Re: jmello]
    #538957 - 03/22/11 04:33 PM (5 years, 8 months ago)

are you talking about microprop? you will have better luck with traditional methods. agar although looks cool creates a whole host of problems for a plant clone.


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OfflineKaptKid
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Registered: 04/20/08
Posts: 5,504
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Re: I'm curious but lost [Re: jmello]
    #538960 - 03/22/11 04:39 PM (5 years, 8 months ago)

Someone tried this before but faded away But there is a thread on it.

Hopefuly Magash will jump in on this one.


--------------------
Child of the 60's, Tripping ever sence.


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Offlinejmello
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Registered: 01/21/10
Posts: 8
Last seen: 1 year, 4 months
Re: I'm curious but lost [Re: KaptKid]
    #539013 - 03/22/11 09:29 PM (5 years, 8 months ago)

Thanks for the replies guys....i mainly want to read up on it....i have a history of trying tough things :smile:

Also I can fit a microprop setup in a very small place...i like stealth  :unabomber:


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OfflineShroomofdoom
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Registered: 10/28/10
Posts: 401
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Re: I'm curious but lost [Re: jmello]
    #539015 - 03/22/11 09:36 PM (5 years, 8 months ago)

I know this is off topic, but are you from Michigan? I had a friend named Justin Mellow (which your name reminds me of) that was from Michigan so im just wondering if thats where you got your name.


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Invisiblemaryanne3087
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Registered: 06/27/10
Posts: 1,111
Trusted Cultivator
Re: I'm curious but lost [Re: jmello]
    #539141 - 03/23/11 08:10 AM (5 years, 8 months ago)

I have this saved on my computer. It was posted at another forum.

INFLUENCE OF CULTIVAR, EXPLANT SOURCE AND PLANT GROWTH
REGULATOR ON CALLUS INDUCTION AND PLANT REGENERATION
OF CANNABIS SATIVA L.
MATERIALS AND METHODS
Seeds of five cultivars of Cannabis sativa L. were
obtained from the Institute of Natural Fibres (Poznan´ ,
Poland): Silesia, Fibrimon-24, Novosadska, Juso-15
and Fedrina-74. Seedlings measuring ~10 cm, grown
in a growth chamber at 22°C under a 12 h photoperiod
were sterilized in 5% calcium hypochlorite for 6, 8 and
15 min and thoroughly rinsed in sterile water. Explants
*e-mail: ajar@igr.poznan.pl
Abbreviations: 2,4-D – dichlorophenoxyacetic acid; KIN – 6-furfurylaminopurine;
NAA – 1-naphthaleneacetic acid; DICAMBA – 3,6-
dichloro-o-anisic acid.
PL ISSN 0001-5296 © Polish Academy of Sciences, Cracow 2005
of young leaves, petioles, internodes and axillary buds
were implanted in Petri dishes (90 × 15 mm) containing
MS basal medium (Murashige and Skoog, 1962) supplemented
with various concentrations of plant growth
substances: 2,4-D, DIC, KIN and NAA (Tab. 1). Growth
regulators were added after the media were autoclaved.
Cultures were kept in darkness at 24°C for 2–3
weeks. Callus was excised from the original explants,
cut into ~0.5 cm3 pieces and transferred every 3 weeks
on the same fresh medium and incubated in a growth
chamber at 22°C under a 16 h photoperiod (~2000 lx).
For root formation, regenerated plantlets ~2 cm high
were excised from callus and cultured on MS basal
medium supplemented with 1.0 mg l-1 IAA and 1.0 mg
l-1 NAA. Rooted plants were transferred to soil and
grown in a greenhouse.


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Offlinejmello
Stranger

Registered: 01/21/10
Posts: 8
Last seen: 1 year, 4 months
Re: I'm curious but lost [Re: maryanne3087]
    #539359 - 03/23/11 09:09 PM (5 years, 8 months ago)

Quote:

Shroomofdoom said:
I know this is off topic, but are you from Michigan? I had a friend named Justin Mellow (which your name reminds me of) that was from Michigan so im just wondering if thats where you got your name.




Sorry man not me :bigjoint: I was high when I came up with that name :pipesmoke:

Quote:

maryanne3087 said:
I have this saved on my computer. It was posted at another forum.

INFLUENCE OF CULTIVAR, EXPLANT SOURCE AND PLANT GROWTH
REGULATOR ON CALLUS INDUCTION AND PLANT REGENERATION
OF CANNABIS SATIVA L.
MATERIALS AND METHODS
Seeds of five cultivars of Cannabis sativa L. were
obtained from the Institute of Natural Fibres (Poznan´ ,
Poland): Silesia, Fibrimon-24, Novosadska, Juso-15
and Fedrina-74. Seedlings measuring ~10 cm, grown
in a growth chamber at 22°C under a 12 h photoperiod
were sterilized in 5% calcium hypochlorite for 6, 8 and
15 min and thoroughly rinsed in sterile water. Explants
*e-mail: ajar@igr.poznan.pl
Abbreviations: 2,4-D – dichlorophenoxyacetic acid; KIN – 6-furfurylaminopurine;
NAA – 1-naphthaleneacetic acid; DICAMBA – 3,6-
dichloro-o-anisic acid.
PL ISSN 0001-5296 © Polish Academy of Sciences, Cracow 2005
of young leaves, petioles, internodes and axillary buds
were implanted in Petri dishes (90 × 15 mm) containing
MS basal medium (Murashige and Skoog, 1962) supplemented
with various concentrations of plant growth
substances: 2,4-D, DIC, KIN and NAA (Tab. 1). Growth
regulators were added after the media were autoclaved.
Cultures were kept in darkness at 24°C for 2–3
weeks. Callus was excised from the original explants,
cut into ~0.5 cm3 pieces and transferred every 3 weeks
on the same fresh medium and incubated in a growth
chamber at 22°C under a 16 h photoperiod (~2000 lx).
For root formation, regenerated plantlets ~2 cm high
were excised from callus and cultured on MS basal
medium supplemented with 1.0 mg l-1 IAA and 1.0 mg
l-1 NAA. Rooted plants were transferred to soil and
grown in a greenhouse.




That actually helps a lot maryanne thank you :smile: I couldn't even really find out what micropropagation involved


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