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OfflineShameMaker
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Registered: 07/13/09
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Loc: Rocky Mountain High State
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Has anyone tried Tissue Cloning yet?
    #318955 - 11/20/09 01:03 AM (7 years, 15 days ago)

One of the more recent High Times mags had an article on tissue cloning.  I was wondering if anyone has tried this yet or if you know any more info on it.  I will soon be running some test runs myself.


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When I feel ashamed I get so scared.  It makes me want to hurt you.


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InvisibleMagashM
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Re: Has anyone tried Tissue Cloning yet? [Re: ShameMaker]
    #318963 - 11/20/09 01:30 AM (7 years, 15 days ago)

It's actually pretty easy. The thing is it's slow. Now if you need huge numbers of clones from only one plant it's the way to go.

http://www.kitchenculturekit.com/Index.htm

http://www.hometissueculture.org/


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All creatures tremble when faced with violence. All creatures fear death, all love life. If we can only see ourselves in others, then how could we possibly hurt another creature?


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Invisiblecaptain.koons
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Registered: 06/25/08
Posts: 6,163
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Re: Has anyone tried Tissue Cloning yet? [Re: Magash]
    #318997 - 11/20/09 02:57 AM (7 years, 15 days ago)

how slow is it?

I want to try this. If it takes less than 2 months I could see it being really viable for SOG growers who don't want to have a clone room.


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TROLLS NEED LOVE TOO!


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InvisibleMagashM
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Re: Has anyone tried Tissue Cloning yet? [Re: captain.koons]
    #319002 - 11/20/09 03:10 AM (7 years, 15 days ago)

It takes pretty close to that 2 months. Even so with a little planning you can still use it  with a sog.


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All creatures tremble when faced with violence. All creatures fear death, all love life. If we can only see ourselves in others, then how could we possibly hurt another creature?


:growingweed: Join us at the Growery! :growingweed:


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InvisibleInverted
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Registered: 06/01/08
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Re: Has anyone tried Tissue Cloning yet? [Re: Magash]
    #319013 - 11/20/09 03:29 AM (7 years, 15 days ago)

:awesome:

If I had the money I'd totally buy one of those kits and give it a shot, I will need lots and lots of sprouts/small plants ready for next years outdoor season :wink:


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InvisibleMagashM
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Re: Has anyone tried Tissue Cloning yet? [Re: Inverted]
    #319024 - 11/20/09 03:44 AM (7 years, 15 days ago)

You have plenty of time to get mother plants going.


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All creatures tremble when faced with violence. All creatures fear death, all love life. If we can only see ourselves in others, then how could we possibly hurt another creature?


:growingweed: Join us at the Growery! :growingweed:


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Invisiblecaptain.koons
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Re: Has anyone tried Tissue Cloning yet? [Re: Inverted]
    #319025 - 11/20/09 03:49 AM (7 years, 15 days ago)

You don't need to buy a kit if you know how to work with agar/sterile mediums.

I want to look into it further. I wonder what is the determinate factor for creating one plant with a root system. I assume a single leaf could be ripped into hundreds of bits and each bit could be turned into a single plant. Making this extremely user friendly and not having like a million tiny sprouts that are unworkable close together would be key.

I imagine if leaf material was used, one could put it into a dropper or pipette and leaf material close enough together could grow into the same plant. I really don't know I'm just making hypotheses.

*Hits google*


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TROLLS NEED LOVE TOO!


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InvisibleInverted
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Re: Has anyone tried Tissue Cloning yet? [Re: Magash]
    #319038 - 11/20/09 05:19 AM (7 years, 15 days ago)

Quote:

Magash said:
You have plenty of time to get mother plants going.





That's what I like to see, at heart i'm really just an outdoor grower.  I just love it so much more for some reason, mother nature takes care of her own...

I love how massive they can get outside too!

You continue to be my hero Magnum Magash!


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Don't criticize what you can't understand


Edited by Inverted (11/20/09 05:19 AM)


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InvisibleDr. SiekadellykM
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Re: Has anyone tried Tissue Cloning yet? [Re: captain.koons]
    #319098 - 11/20/09 12:13 PM (7 years, 14 days ago)

wow, i had no idea you could tissue clone a plant like shrooms?
thats awesome i want to try it.


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InvisibleTriptonic
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Registered: 06/14/08
Posts: 15,581
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Re: Has anyone tried Tissue Cloning yet? [Re: Dr. Siekadellyk]
    #319100 - 11/20/09 12:17 PM (7 years, 14 days ago)

Quote:

Dr. Siekadellyk said:
wow, i had no idea you could tissue clone a plant like shrooms?
thats awesome i want to try it.



Agreed.


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InvisibleInverted
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Re: Has anyone tried Tissue Cloning yet? [Re: Triptonic]
    #319112 - 11/20/09 12:40 PM (7 years, 14 days ago)

Yep, it's a pretty awesome deal, making thousands of potential clones from a small plant/part of the plant.  I'd almost rather use this before conventional cuttings IF I had the time...


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OfflineShameMaker
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Registered: 07/13/09
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Loc: Rocky Mountain High State
Last seen: 1 day, 20 hours
Re: Has anyone tried Tissue Cloning yet? [Re: captain.koons]
    #319116 - 11/20/09 01:14 PM (7 years, 14 days ago)

Quote:

captain.koons said:
You don't need to buy a kit if you know how to work with agar/sterile mediums.

It is possible to make thousands of clones from a single plant.

Yeah u use agar as a medium and u need a glove box (Its real similar to mushroom cloning.) there r 3 main hormones u need to add to the agar. but the trickiest part indefinitely is the sterile work that needs to be done.  If u can do it right, u can have all the plants u need to last you through the year all contained in one small closet.  It doesnt get any more convenient than that.


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When I feel ashamed I get so scared.  It makes me want to hurt you.


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InvisibleFurrowedBrowM
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Re: Has anyone tried Tissue Cloning yet? [Re: ShameMaker]
    #319169 - 11/20/09 03:34 PM (7 years, 14 days ago)

very interesting.  I've got 500 petri dishes in the other room that have needed some action for a while now.


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Multidisciplinary Association for Psychedelic Studies - Become a member!
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OfflineFarmer Joe
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Re: Has anyone tried Tissue Cloning yet? [Re: FurrowedBrow]
    #319339 - 11/20/09 09:04 PM (7 years, 14 days ago)

Nice link magash. I had never heard of this method of cloning until reading this post. I didn't have time to read the entire article but I plan to when I get outta work.

Hey Shamemaker:

Do you plan on documenting your tissue cloning "test run" in a grow log here at the growery?
I hope you do. I am very intrigued by this post!  I can't wait to get  home do more research.

                                                                                                                                        :peace: FJ


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"Marijuana may not be addictive, but growing it is" - ED Rosenthal



Maine Caregiver In 100% compliance with Maine state laws.


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InvisibleMagashM
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Re: Has anyone tried Tissue Cloning yet? [Re: Farmer Joe]
    #319387 - 11/20/09 10:34 PM (7 years, 14 days ago)

It really is much easier then you think. I'm telling ya anybody can do it. :thumbup:


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All creatures tremble when faced with violence. All creatures fear death, all love life. If we can only see ourselves in others, then how could we possibly hurt another creature?


:growingweed: Join us at the Growery! :growingweed:


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OfflineShameMaker
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Registered: 07/13/09
Posts: 35
Loc: Rocky Mountain High State
Last seen: 1 day, 20 hours
Re: Has anyone tried Tissue Cloning yet? [Re: Farmer Joe]
    #319951 - 11/22/09 04:13 PM (7 years, 12 days ago)

Yeah, ill go ahead and keep you updated on my situation and post a thread in the grow journals.  Keep in mind that i'm a small-time closet grower and im starting some new plants within the next week or two. So ill be trying to make a couple of clones for each plant i get going(probably 4-6). But i still need to get the proper growth hormones. Included is a pic of my homemade glove box.  Sterility is the key!



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When I feel ashamed I get so scared.  It makes me want to hurt you.


Edited by ShameMaker (11/22/09 04:13 PM)


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Offline81renaissance
Coachella '13 KKOTY
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Re: Has anyone tried Tissue Cloning yet? [Re: ShameMaker]
    #319961 - 11/22/09 04:27 PM (7 years, 12 days ago)

:lol: that box takes me back to the shroomie days.
Its been a minute since I cultivated fungus. :sad:


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"So it goes."
-Kurt Vonnegut


BlueBerry_Swisher said:I want French fries. No, I want a penis French. Thank you. I'm so excited. I can not contain myself. Now I eat chocolate. It is so good. I'm trying to rub it all over myself. And then lick. Now I need a hot shower. The end.


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OfflineFarmer Joe
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Re: Has anyone tried Tissue Cloning yet? [Re: 81renaissance]
    #320144 - 11/22/09 09:34 PM (7 years, 12 days ago)

nice lil lab you got there. I look forward to your log  :goodluck:


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"Marijuana may not be addictive, but growing it is" - ED Rosenthal



Maine Caregiver In 100% compliance with Maine state laws.


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InvisibleFurrowedBrowM
Free yourself from yourself
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Re: Has anyone tried Tissue Cloning yet? [Re: Farmer Joe]
    #327395 - 12/05/09 04:15 PM (6 years, 11 months ago)

I just found this thread over at icmag on the subject.  it's worth a read.


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Multidisciplinary Association for Psychedelic Studies - Become a member!
The Growery's Herb Museum (post #24)
I prefer dangerous freedom to peaceful slavery.
~ Thomas Jefferson ~


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