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 strangladesh
Stranger
Registered: 01/15/11
Posts: 55
Last seen: 11 years, 3 months
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 marijuana microproagation 6-bap for callus 
#534818 - 03/08/11 05:55 PM (13 years, 1 month ago) |
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Should i use 6-bap for callusing. how many ppm? What is a good recipe.
can some one help me out?
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 Harry_Ba11sach
cannoisseur
Registered: 04/20/08
Posts: 11,753
Loc: Nepal 
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Re: marijuana microproagation 6-bap for callus [Re: strangladesh]
#534820 - 03/08/11 05:56 PM (13 years, 1 month ago) |
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Edit: after some research, 6-Bap sounds incredibly interesting. PLEASE try it on a crop and let us know (PICTURES)
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DungenessDank
Lord of the Flies
Registered: 05/05/08
Posts: 9,372
Loc: PNW
Last seen: 10 years, 11 months
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Re: marijuana microproagation 6-bap for callus [Re: Harry_Ba11sach]
#535066 - 03/09/11 06:32 AM (13 years, 1 month ago) |
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Quote:
Harry_Ba11sach said:
Edit: after some research, 6-Bap sounds incredibly interesting. PLEASE try it on a crop and let us know (PICTURES)
2nd'd
If you are going to attempt a home micro-prop post pictures!
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Harry_Ba11sach
cannoisseur
Registered: 04/20/08
Posts: 11,753
Loc: Nepal
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Re: marijuana microproagation 6-bap for callus [Re: strangladesh]
#535097 - 03/09/11 09:35 AM (13 years, 1 month ago) |
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Quote:
strangladesh said:
Should i use 6-bap for callusing. how many ppm? What is a good recipe.
can some one help me out?
However, I should add that you DO NOT WANT callusing. Calluses are undifferentiated tissue and in absolutely no way are the beneficial for you as a grower.
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the man
Registered: 04/20/08
Posts: 825
Last seen: 11 years, 3 months
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Re: marijuana microproagation 6-bap for callus [Re: Harry_Ba11sach]
#535100 - 03/09/11 09:41 AM (13 years, 1 month ago) |
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it is if you want to proliferate or keep a petri sized mother. also can sent wedges of callus pretty easily for perfect clones in small area 
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Harry_Ba11sach
cannoisseur
Registered: 04/20/08
Posts: 11,753
Loc: Nepal
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Re: marijuana microproagation 6-bap for callus [Re: the man]
#535101 - 03/09/11 09:42 AM (13 years, 1 month ago) |
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Yes, like I was saying, completely useless for your standard grower. Have you ever seen a professional microprop setup?
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the man
Registered: 04/20/08
Posts: 825
Last seen: 11 years, 3 months
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Re: marijuana microproagation 6-bap for callus [Re: Harry_Ba11sach]
#535113 - 03/09/11 10:12 AM (13 years, 1 month ago) |
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yup, my opnion microprop is useless unless you do callus go figure
i think for smaller growers would be the best. want to do dif strains but keep a mother but dont have space. cool stuff if you can get ahold of the hormones etc
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Harry_Ba11sach
cannoisseur
Registered: 04/20/08
Posts: 11,753
Loc: Nepal
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Re: marijuana microproagation 6-bap for callus [Re: the man]
#535114 - 03/09/11 10:16 AM (13 years, 1 month ago) |
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well of course it's useless unless you callus, that's what I'm saying. Almost NO private citizens have access to the plethora of hormones, tissue culturing agar plates, and extreme climate control chambers necessary to adequately utilize the technique. Not to mention the insane amount of time it takes to get perfectly set up.
Would be sweet for the cops to bust someone with 200 strains suspended in microprop fashion though. They would have no idea what they're looking at.
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the man
Registered: 04/20/08
Posts: 825
Last seen: 11 years, 3 months
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Re: marijuana microproagation 6-bap for callus [Re: Harry_Ba11sach]
#535197 - 03/09/11 02:36 PM (13 years, 1 month ago) |
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ya i mean there is optimum conditions like in mushroom cult then there is the very workable teks peopel come up with. but if kits or hormones where easy to get i think its awesome to try!
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Harry_Ba11sach
cannoisseur
Registered: 04/20/08
Posts: 11,753
Loc: Nepal
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Re: marijuana microproagation 6-bap for callus [Re: the man]
#535198 - 03/09/11 02:38 PM (13 years, 1 month ago) |
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I would support it.
I think I'm going to buy some 6-BAP here pretty soon and give it a try on a variety of vegetables. I don't like that it's synthetic, but if it's truly a cytokinin then it should be perfectly healthy for consumption since cytokinins of many different forms are in literally every plant anyone on the planet has ever eaten.
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the man
Registered: 04/20/08
Posts: 825
Last seen: 11 years, 3 months
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Re: marijuana microproagation 6-bap for callus [Re: Harry_Ba11sach]
#535202 - 03/09/11 02:54 PM (13 years, 1 month ago) |
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you still want to play safe with plant hormones synthed or not esp when concentrated. do it up man keep us posted. is there orchid cloning kits ?
http://www.nutraingredients.com/Research/Breakthrough-for-research-on-plant-hormone-effects
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Dr. Penguin
Registered: 05/19/08
Posts: 1,036
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Re: marijuana microproagation 6-bap for callus [Re: the man]
#536257 - 03/12/11 11:36 AM (13 years, 1 month ago) |
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Wow, awesome question, its great to see people interested in micro-prop. I actually have a very well made pdf about the callus culture of cannabis but unfortunately I'm on vacation so I don't have it with me.
Quote:
Almost NO private citizens have access to the plethora of hormones, tissue culturing agar plates, and extreme climate control chambers necessary to adequately utilize the technique. Not to mention the insane amount of time it takes to get perfectly set up.
I don't know if I agree with you harry. I personally have everything I need to induce and set a callus grown plantlet and I'm sure a couple others on this board do as well. In most plants it is relatively trivial to induce a callus to form requiring only a light source, petri dishes, bleach, MS media, and maybe a set of 2-3 hormones to induce root and shoot formation. The biggest challenge in doing this type of propagation is keeping it sterile as fungi and bacteria will try to out-compete the plant for nutrients.
The biggest drawback with this technique is the time required to generate the necessary clones. It can take a couple months to go from a callus to a plantlet that is hardened off and ready to plant. On the other hand, using it as a genetic storage technique is something well within the reach of a home cultivator.
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the man
Registered: 04/20/08
Posts: 825
Last seen: 11 years, 3 months
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Re: marijuana microproagation 6-bap for callus [Re: Dr. Penguin]
#536365 - 03/12/11 05:45 PM (13 years, 1 month ago) |
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so where and how affordable were the hormones ??
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the man
Registered: 04/20/08
Posts: 825
Last seen: 11 years, 3 months
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Re: marijuana microproagation 6-bap for callus [Re: the man]
#536382 - 03/12/11 06:30 PM (13 years, 1 month ago) |
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Harry_Ba11sach
cannoisseur
Registered: 04/20/08
Posts: 11,753
Loc: Nepal
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Re: marijuana microproagation 6-bap for callus [Re: Dr. Penguin]
#536383 - 03/12/11 06:31 PM (13 years, 1 month ago) |
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Quote:
Dr. Penguin said:
Wow, awesome question, its great to see people interested in micro-prop. I actually have a very well made pdf about the callus culture of cannabis but unfortunately I'm on vacation so I don't have it with me.
Quote:
Almost NO private citizens have access to the plethora of hormones, tissue culturing agar plates, and extreme climate control chambers necessary to adequately utilize the technique. Not to mention the insane amount of time it takes to get perfectly set up.
I don't know if I agree with you harry. I personally have everything I need to induce and set a callus grown plantlet and I'm sure a couple others on this board do as well. In most plants it is relatively trivial to induce a callus to form requiring only a light source, petri dishes, bleach, MS media, and maybe a set of 2-3 hormones to induce root and shoot formation. The biggest challenge in doing this type of propagation is keeping it sterile as fungi and bacteria will try to out-compete the plant for nutrients.
The biggest drawback with this technique is the time required to generate the necessary clones. It can take a couple months to go from a callus to a plantlet that is hardened off and ready to plant. On the other hand, using it as a genetic storage technique is something well within the reach of a home cultivator.
Fair enough. I suppose I'm not used to people attempting to micro-prop with civilian setups. All my work with that has been in frighteningly expensive laboratories with extremely rigorous climate controls.
Any pics/more details of your setup?
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canndo
Stranger
Registered: 03/23/11
Posts: 7
Last seen: 13 years, 1 month
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Re: marijuana microproagation 6-bap for callus [Re: strangladesh]
#539254 - 03/23/11 02:27 PM (13 years, 1 month ago) |
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I have found that Dicamba is your best bet for callus production in Cannibis S. But I am curious as to why you would want to produce Callus. I never had a problem establishing roots from callus with C. S. but there is the problem, I have yet to generate a shoot. Everything I have read indicates that shoot production is very very low.
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canndo
Stranger
Registered: 03/23/11
Posts: 7
Last seen: 13 years, 1 month
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Re: marijuana microproagation 6-bap for callus [Re: canndo]
#539258 - 03/23/11 02:37 PM (13 years, 1 month ago) |
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I have been doing micropropagation of our favorite plant for quite some time now. I will post pictures when I can reduce the size of them for this site. It is a myth that you need thousands and thousands of dollars to get started. It is a myth that sterility is an issue - if you can grow mushrooms, you are likely already versed enough in sterile techniques to do this.
I have done transfers and such in a room with still air only. Using a decent biocide in your medium helps greatly and I swear by such techniques. I have designed a very decent establishment protocol and a good multiplication protocol as well. The chemicals, although expensive because you have to buy in bulk, are cheap because you use so damn little. Most of them are heat and/or light sensitive and one requires over night shipping but a black bag and a refigerator is all that is needed to keep things safe.
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the man
Registered: 04/20/08
Posts: 825
Last seen: 11 years, 3 months
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Re: marijuana microproagation 6-bap for callus [Re: canndo]
#539267 - 03/23/11 02:53 PM (13 years, 1 month ago) |
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well wonder if stronger hormone mix will make the shoot come faster or is it just a waiting game? pics!!
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canndo
Stranger
Registered: 03/23/11
Posts: 7
Last seen: 13 years, 1 month
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Re: marijuana microproagation 6-bap for callus [Re: the man]
#539268 - 03/23/11 02:55 PM (13 years, 1 month ago) |
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No, more is definitely not better, it is the balance that makes all the difference.
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canndo
Stranger
Registered: 03/23/11
Posts: 7
Last seen: 13 years, 1 month
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Re: marijuana microproagation 6-bap for callus [Re: canndo]
#539270 - 03/23/11 02:59 PM (13 years, 1 month ago) |
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You can indeed generate shoots from shoots - I have been doing it for well over a year now. My pictures are all 1meg, this site demands .5 meg, is that correct?
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canndo
Stranger
Registered: 03/23/11
Posts: 7
Last seen: 13 years, 1 month
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Re: marijuana microproagation 6-bap for callus [Re: canndo]
#539272 - 03/23/11 03:14 PM (13 years, 1 month ago) |
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I'll work on it later, I am at a slight disadvantage as I winnowed out all of my plantlets two days ago so I can't show pictures of lush little plantlets in jars. You can see a single recently cut shoot in my avatar picture.
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Tank333
Psychotic Hippie
Registered: 08/19/10
Posts: 1,241
Loc: Washington
Last seen: 4 years, 3 months
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Re: marijuana microproagation 6-bap for callus [Re: canndo]
#539274 - 03/23/11 03:24 PM (13 years, 1 month ago) |
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Can't wait to see pics. This sounds VERY interesting, and I'd love to give it a whirl once I'm more experienced!
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My best run so far
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Dr. Penguin
Registered: 05/19/08
Posts: 1,036
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Re: marijuana microproagation 6-bap for callus [Re: Tank333]
#541328 - 03/29/11 03:56 PM (13 years, 29 days ago) |
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Heres the book I said I'd post. It's pretty neat stuff, have a look!
The Biotechnology of C. sativa
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kyuzo
Stranger Than Fiction
Registered: 07/05/10
Posts: 981
Last seen: 11 years, 3 months
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Re: marijuana microproagation 6-bap for callus [Re: Dr. Penguin]
#541355 - 03/29/11 05:05 PM (13 years, 29 days ago) |
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kyuzo
Stranger Than Fiction
Registered: 07/05/10
Posts: 981
Last seen: 11 years, 3 months
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Re: marijuana microproagation 6-bap for callus [Re: Dr. Penguin]
#541357 - 03/29/11 05:08 PM (13 years, 29 days ago) |
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Quote:
Dr. Penguin said:
Heres the book I said I'd post. It's pretty neat stuff, have a look!
The Biotechnology of C. sativa
thanks for posting that
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DungenessDank
Lord of the Flies
Registered: 05/05/08
Posts: 9,372
Loc: PNW
Last seen: 10 years, 11 months
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Re: marijuana microproagation 6-bap for callus [Re: Tank333]
#541479 - 03/29/11 10:28 PM (13 years, 29 days ago) |
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Quote:
Tank333 said:
Can't wait to see pics. This sounds VERY interesting, and I'd love to give it a whirl once I'm more experienced!
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Tank333
Psychotic Hippie
Registered: 08/19/10
Posts: 1,241
Loc: Washington
Last seen: 4 years, 3 months
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Re: marijuana microproagation 6-bap for callus [Re: DungenessDank]
#541480 - 03/29/11 10:31 PM (13 years, 29 days ago) |
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I am even more intrigued! I have an 8 hour car ride tomorrow, so I'll probably have time to read the whole thing...
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My best run so far
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kyuzo
Stranger Than Fiction
Registered: 07/05/10
Posts: 981
Last seen: 11 years, 3 months
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Re: marijuana microproagation 6-bap for callus [Re: Tank333]
#542987 - 04/04/11 04:12 AM (13 years, 23 days ago) |
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Just thought I would add these resources
the first is a website is an organization that does seminars (they are going to be in Washington this summer) and sell cheap supplies, including hormones in small amounts
http://www.hometissueculture.org/
second is their youtube channel
http://www.youtube.com/user/fbt2007
third is their yahoo discussion group
http://tech.groups.yahoo.com/group/hometissueculture/
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OverDoseLiving
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Re: marijuana microproagation 6-bap for callus [Re: kyuzo]
#543257 - 04/04/11 07:56 PM (13 years, 23 days ago) |
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Whats the difference between micro propagation an just aking small clones? Are there any advantages?? I tried my hand at 'tissue culturing; small half inch and sub half inch shoots but failed on all but the largest ones due to mold, and the rest never really took off for some reason...
How big do most clippings have to be in orer to be rooted?
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petridish
Stranger
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Re: marijuana microproagation 6-bap for callus [Re: Dr. Penguin]
#611030 - 02/29/12 03:09 PM (12 years, 1 month ago) |
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I can't seem to look at this book. I clicked on it and it's been loading for abt 30 minutes, but nothing is coming up on the page. Is it maybe not still around?
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petridish
Stranger
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Re: marijuana microproagation 6-bap for callus [Re: petridish]
#611031 - 02/29/12 03:12 PM (12 years, 1 month ago) |
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My bad, it finished loading while I posted.
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