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OfflineShameMaker
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Registered: 07/13/09
Posts: 35
Loc: Rocky Mountain High State
Last seen: 2 days, 20 hours
First Tissue Cloning Trials
    #320040 - 11/22/09 06:35 PM (7 years, 13 days ago)

Okay I am planning on trying to clone some plants using this new tissue cloning technique.  For those who haven't heard its the process of taking a fragment of a piece of leaf or stem and turning it into an entire plant. I am just now starting to germinate 6 seeds that i will attempt to clone(2xSkunk, 2xBlueVelvet, 2xBubbleGum)

This trial and error begins with new plants.  Once they're a couple of weeks old and have about 4 leaves i will snip a solitary leaf off, from each plant, and prepare it to be cloned.  Since it takes only a fraction of a leaf ill try and make 3 clones from each leaf.  Once they are sterile I will put them into their first jars of agar.

So far i'm not too sure what to use in my agar.  I will use the standard agar method(used for mushroom culture) which consist of agar agar, potato flakes, and honey.  I'm also going to try and find the growth hormones NAA and BA for this medium.  Ba is a hormone that promotes rooting.

A sterile work environment is vital.  I am using a homemade glove box for this task. 

I won't have any real information ready for this anytime real soon but I will try and keep you informed of everything that happens as it comes.

If anyone has any suggestions or thinks im going about this set up wrongly please let me know.


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When I feel ashamed I get so scared.  It makes me want to hurt you.


Edited by ShameMaker (11/22/09 06:40 PM)


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OfflineSmOakland
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Registered: 05/31/09
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Re: First Tissue Cloning Trials [Re: ShameMaker]
    #320051 - 11/22/09 06:48 PM (7 years, 13 days ago)

Sorry but is there a reason why you are trying this or are you just curious?


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OfflineShameMaker
Male


Registered: 07/13/09
Posts: 35
Loc: Rocky Mountain High State
Last seen: 2 days, 20 hours
Re: First Tissue Cloning Trials [Re: SmOakland]
    #320059 - 11/22/09 06:59 PM (7 years, 13 days ago)

curiosity mainly? i dont know what that has to do with anything. I mean this is a grow journal right? i am merely documenting my tissue cloning trials so people can read and run trials themselves.  Someone asked me to keep a record of these tissue clone trials.  If it works i will help explain what you need to know to do it yourself.  i have a very limited number of some dank seeds left.  i am trying to figure out this whole tissue cloning thing so i can clone my own plants and keep my dank skunk strain alive.  Tissue cloning, if done right, is supposed to be convenient.  i would like to have the power and option of taking one simple leaf from another growers plant and have the exact same genetics. 

If you know more about this then please enlighten me with some ideas.


--------------------

When I feel ashamed I get so scared.  It makes me want to hurt you.


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OfflineSmOakland
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Re: First Tissue Cloning Trials [Re: ShameMaker]
    #320062 - 11/22/09 07:02 PM (7 years, 13 days ago)

I just wanted to know if there were any advantages to cloning this way.


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OfflineShameMaker
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Registered: 07/13/09
Posts: 35
Loc: Rocky Mountain High State
Last seen: 2 days, 20 hours
Re: First Tissue Cloning Trials [Re: SmOakland]
    #320068 - 11/22/09 07:07 PM (7 years, 13 days ago)

O well of course!  you can make thousands(literally) of clones from a single plant(in its vegetative phase). but not only can u make an infinite number of clones, u can contain them in small jars.  this terminates the mother plant strategy.  No longer keeping a mother plant in veggie stage all the time.  You can easily have 500 copies of the exact same plant in a closet on one shelf. stored and ready to be transplanted when you are.


--------------------

When I feel ashamed I get so scared.  It makes me want to hurt you.


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InvisibleDr. SiekadellykM
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Re: First Tissue Cloning Trials [Re: ShameMaker]
    #320085 - 11/22/09 07:42 PM (7 years, 13 days ago)

:thumbup:I can't wait i need to see this!:banana:


--------------------
The Kratom Report...


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OfflineDudeTron
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Registered: 10/24/09
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Re: First Tissue Cloning Trials [Re: Dr. Siekadellyk]
    #320114 - 11/22/09 08:39 PM (7 years, 13 days ago)

I was doing a little reading about this.

Have you considered using a part of the plant containing the fastest dividing cells?  As in, perhaps a piece of new growth tip on a lower node or something.  The highest density of rapidly growing stuff.... dig?

Here's a TC recipe I found that seems pretty generic:

1/2 teaspoon Peter's fertilizer (20-20-20)
2 tablespoon sugar
1 multi-vitamin pill
1 - 2 ml PPM (optional but very hard without it) 
Agar or gelatin (I've never used gelatin)
4 cups Bottled or RO Sodium Free Water
Vinegar or Baking Soda to adjust pH

Here's a link to that PPM stuff mentioned above, kinda interesting: http://www.ppm4plant-tc.com/

So, there's a couple more considerations for you... fertilizer would apparently be acceptable in your media, and also the PH should probably be adjusted...

Good luck, I'll be watching this one


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OfflineFarmer Joe
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Registered: 09/03/09
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Re: First Tissue Cloning Trials [Re: DudeTron]
    #320154 - 11/22/09 09:51 PM (7 years, 13 days ago)

Nice to see you didn't waste any time.  I've got my popcorn ready.


--------------------
"Marijuana may not be addictive, but growing it is" - ED Rosenthal



Maine Caregiver In 100% compliance with Maine state laws.


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OfflineShameMaker
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Registered: 07/13/09
Posts: 35
Loc: Rocky Mountain High State
Last seen: 2 days, 20 hours
Re: First Tissue Cloning Trials [Re: DudeTron]
    #320197 - 11/22/09 10:58 PM (7 years, 13 days ago)

thanks for your research. Ima go ahead and reconstruct my agar media based on the info youve provided.  im also assuming i can use distilled water besides RO water.  and yeah ill definitely use the newest leaflets from each plant. I was going to wait and do this in a few more weeks but farmer joe had asked me to start this grow journal so i decided to go ahead and get a move on with it.  Assuming that this works the first time or two around it will be several months before it is completely finished and full of results. ty for your support


--------------------

When I feel ashamed I get so scared.  It makes me want to hurt you.


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OfflineSmOakland
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Re: First Tissue Cloning Trials [Re: ShameMaker]
    #320338 - 11/23/09 01:11 AM (7 years, 13 days ago)

Sorry to be a lazy ass but can someone post a link to an overview of this process?


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OfflineShameMaker
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Registered: 07/13/09
Posts: 35
Loc: Rocky Mountain High State
Last seen: 2 days, 20 hours
Re: First Tissue Cloning Trials [Re: SmOakland]
    #320343 - 11/23/09 01:29 AM (7 years, 13 days ago)



--------------------

When I feel ashamed I get so scared.  It makes me want to hurt you.


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Invisibledr_gonz

Registered: 01/31/09
Posts: 137
Re: First Tissue Cloning Trials *DELETED* [Re: ShameMaker]
    #321225 - 11/24/09 09:50 PM (7 years, 11 days ago)

Post deleted by dr_gonz

Reason for deletion: a



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InvisibleInverted
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Registered: 06/01/08
Posts: 9,953
Loc: North Star Flag
Re: First Tissue Cloning Trials [Re: dr_gonz]
    #321276 - 11/24/09 11:25 PM (7 years, 11 days ago)

I think a lot of people will be watching this thread.

I wish I had the funds or I would invest in this method, as I'm starting to grow on the idea of Sea Of Green.  I like small, fast flowering plants, and in high numbers you can yield very high.

That's awesome that you have the balls to dive right into a new method of cloning.  I hear patience is a virtue.

Good luck! :yesnod:

Inverted


--------------------
Don't criticize what you can't understand


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OfflineShameMaker
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Loc: Rocky Mountain High State
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Re: First Tissue Cloning Trials [Re: Inverted]
    #321290 - 11/25/09 12:15 AM (7 years, 11 days ago)

All I can do is try. I honestly don't expect great results the first time around but I will let people know what i am doing either way so they can make their own conclusions based on my findings.  So far I've put together a rough media that i will be testing but chances are this won't be the right one.  Ive got to go get more supplies sometime after thanksgiving.  Also trying to sprout some new seeds but nothing yet.  I'll keep you guys posted. Wish me luck!


--------------------

When I feel ashamed I get so scared.  It makes me want to hurt you.


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OfflineKaptKid
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Re: First Tissue Cloning Trials [Re: ShameMaker]
    #321307 - 11/25/09 12:51 AM (7 years, 11 days ago)

Best of luck to you.

I'll be watching this thread.:popcorn:


I need 60 clones for my next grow. Have space for another 60.
Got to do that walk before you run thing.


--------------------
Child of the 60's, Tripping ever sence.


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Invisibledr_gonz

Registered: 01/31/09
Posts: 137
Re: First Tissue Cloning Trials *DELETED* [Re: KaptKid]
    #321377 - 11/25/09 08:42 AM (7 years, 11 days ago)

Post deleted by dr_gonz

Reason for deletion: z



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OfflineDudeTron
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Registered: 10/24/09
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Re: First Tissue Cloning Trials [Re: dr_gonz]
    #321387 - 11/25/09 10:45 AM (7 years, 11 days ago)

What media mix did you end up using?


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OfflineFarmer Joe
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Re: First Tissue Cloning Trials [Re: DudeTron]
    #324878 - 12/01/09 10:22 PM (7 years, 4 days ago)

any updates?


--------------------
"Marijuana may not be addictive, but growing it is" - ED Rosenthal



Maine Caregiver In 100% compliance with Maine state laws.


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Invisiblecoda


Registered: 04/20/08
Posts: 4,734
Trusted Cultivator
Re: First Tissue Cloning Trials [Re: Farmer Joe]
    #327467 - 12/05/09 07:13 PM (7 years, 16 hours ago)

yah, hope to hear something new soon.  Good luck with this, I'm interested to see how this all turns out!


--------------------


MFDoom666: sobriety kills my buzz every time.


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Invisibledr_gonz

Registered: 01/31/09
Posts: 137
Re: First Tissue Cloning Trials *DELETED* [Re: coda]
    #341576 - 01/06/10 03:41 PM (6 years, 10 months ago)

Post deleted by dr_gonz

Reason for deletion: .



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