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OfflineC3R3AL KiLLRx
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Agar Question
    #271954 - 08/29/09 09:23 AM (12 years, 9 months ago)

can i transfer an agar slice to a brf pf tek style jar?


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InvisibleDr. SiekadellykM
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Re: Agar Question [Re: C3R3AL KiLLRx]
    #271968 - 08/29/09 11:00 AM (12 years, 9 months ago)

I would imagine its possible but I'm not sure how high the risk of contamination is....

Aren't you signed up at the shroomery also? you'd probably get a more accurate and faster answer over there.


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OfflineC3R3AL KiLLRx
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Re: Agar Question [Re: Dr. Siekadellyk]
    #272021 - 08/29/09 02:11 PM (12 years, 9 months ago)

i have a glovebox and i did some research and yea itll work


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InvisibleDr. SiekadellykM
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Re: Agar Question [Re: C3R3AL KiLLRx]
    #272304 - 08/29/09 09:19 PM (12 years, 9 months ago)

:thumbup:


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OfflineC3R3AL KiLLRx
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Re: Agar Question [Re: Dr. Siekadellyk]
    #272309 - 08/29/09 09:28 PM (12 years, 9 months ago)

:thumbup:


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OfflineC3R3AL KiLLRx
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Re: Agar Question [Re: C3R3AL KiLLRx]
    #272311 - 08/29/09 09:29 PM (12 years, 9 months ago)

So now i have 6 Eq's sliced with agar 3 penis envy (spore innoced) and 3 Huatla(spore inocced also)


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InvisibleFurrowedBrowM
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Re: Agar Question [Re: C3R3AL KiLLRx]
    #274961 - 09/03/09 08:29 PM (12 years, 9 months ago)

PF jars usually contain a top layer of verm for contamination protection.  You would have to get the myc on the agar down onto the sides down farther like where the spores drain down to when you inject with a needle.  You can do the same thing with a print.  How many isolations did you do?


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OfflineC3R3AL KiLLRx
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Re: Agar Question [Re: FurrowedBrow]
    #275259 - 09/04/09 06:24 PM (12 years, 9 months ago)

10xisolated eq theyre sooooo rhizomorphic and theyres already alot of colonization i used a gloveboxto prevent contam i used the top layer only on the jars i innoculated iam no newb just didnt know much about growing from agar just fucked with it for a while till i got theses ill post pix in a min..


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OfflineC3R3AL KiLLRx
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Re: Agar Question [Re: C3R3AL KiLLRx]
    #275264 - 09/04/09 06:41 PM (12 years, 9 months ago)



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InvisibleFurrowedBrowM
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Re: Agar Question [Re: C3R3AL KiLLRx]
    #275285 - 09/04/09 07:58 PM (12 years, 9 months ago)

Looks like a good one!  What's with the rippled edge on the dish?


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OfflineC3R3AL KiLLRx
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Re: Agar Question [Re: FurrowedBrow]
    #275286 - 09/04/09 08:12 PM (12 years, 9 months ago)

micro pore tape


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InvisibleFurrowedBrowM
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Re: Agar Question [Re: C3R3AL KiLLRx]
    #275288 - 09/04/09 08:13 PM (12 years, 9 months ago)

oh gotcha!  you have a log for these?


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OfflineC3R3AL KiLLRx
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Re: Agar Question [Re: FurrowedBrow]
    #275294 - 09/04/09 08:23 PM (12 years, 9 months ago)

ima wait and post all at once...and another question i used red seaweed flakes as the medium and I also kncked up a LC with the same Iso...do you think after re hydration the flakes would turn red again cuz there is red flakes in it but the jars are growing fine and i did under the same sterile conditions.


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InvisibleFurrowedBrowM
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Re: Agar Question [Re: C3R3AL KiLLRx]
    #275296 - 09/04/09 08:27 PM (12 years, 9 months ago)

I've never heard of red seaweed flakes.  This is your substrate for what?


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OfflineC3R3AL KiLLRx
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Re: Agar Question [Re: FurrowedBrow]
    #275300 - 09/04/09 08:38 PM (12 years, 9 months ago)

the agar beleive it or not


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InvisibleFurrowedBrowM
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Re: Agar Question [Re: C3R3AL KiLLRx]
    #275306 - 09/04/09 09:07 PM (12 years, 9 months ago)

oh ok.  That is something I am not sure of.  Better to ask the advanced mycology ppls.  Here's an experiement I started a few years ago.  I didn't fruit it though.  Maybe I should try it again. 

http://www.shroomery.org/forums/showflat.php/Number/7741291#7741291


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OfflineC3R3AL KiLLRx
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Re: Agar Question [Re: FurrowedBrow]
    #275309 - 09/04/09 09:30 PM (12 years, 9 months ago)

lol minty or any red lol?


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InvisibleFurrowedBrowM
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Re: Agar Question [Re: C3R3AL KiLLRx]
    #275312 - 09/04/09 09:35 PM (12 years, 9 months ago)

They didn't get fruited.  :frown:


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OfflineC3R3AL KiLLRx
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Re: Agar Question [Re: FurrowedBrow]
    #275393 - 09/05/09 08:53 AM (12 years, 9 months ago)

:frown:


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Re: Agar Question [Re: C3R3AL KiLLRx]
    #275677 - 09/06/09 11:14 AM (12 years, 9 months ago)

The best way to use an agar wedge in a PF Tek style jar is to change the dry verm filter for micropore tape on top of the jar. Lots of people do it this way to test their isolates. :laugh:

:peace:


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